Fig. 5From: Total RNA extraction from tissues for microRNA and target gene expression analysis: not all kits are created equalQuantitative real-time PCR analyses of target gene expression from murine tissues. EGFR and AXL expression was analysed from the same RNA samples isolated from brain (a), liver (b) and lung (c) tissues for miRNA detection. Target gene expression was normalised to either: B2M and 18S/HPRT as reference genes. Values indicate the average normalised gene expression (technical duplicates from triplicate isolations) in different tissues on a Log2 scale with SEM error bars. * p < 0.05 when compared to three or more other methodsBack to article page