Fig. 1From: Total RNA extraction from tissues for microRNA and target gene expression analysis: not all kits are created equalExperimental design for comparing RNA isolation methods from mouse tissues. Brain, lung and liver tissues were dissected from C57BL/6 mice (n = 3) and rapidly frozen. Tissues were homogenised and RNA extracted using five different methods and assessed for yield, purity, integrity and miRNA abundance. miRNA and target gene expression was then quantitated with qRT-PCRBack to article page