Sr. No. | Enzymes | K m (mg/ml) | K cat (min−1) | Kcat/Km (ml min−1 mg−1) |
---|---|---|---|---|
1. | rBAxn | 19.34 ± 0.72 | 6449.12 ± 155.37 | 333.83 ± 6.78 |
2. | W63A | * | * | * |
3. | Y128A | * | * | * |
4. | W144A | * | * | * |
5. | Y124A | 23.92 ± 0.10 | 823.58 ± 3.12 | 34.81 ± 0.19 |
6. | W187A | 140.36 ± 1.15 | 4258.08 ± 89.73 | 28.75 ± 0.61 |
7. | W187A_Y124A | 45.75 ± 0.23 | 1080.65 ± 23.65 | 23.62 ± 0.26 |