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Table 4 Kinetic parameters of rBAxn and engineered enzymes using 1% beechwood xylan as a substrate

From: Effects of substrate binding site residue substitutions of xynA from Bacillus amyloliquefaciens on substrate specificity

Sr. No.

Enzymes

K m

(mg/ml)

K cat

(min−1)

Kcat/Km

(ml min−1 mg−1)

1.

rBAxn

19.34 ± 0.72

6449.12 ± 155.37

333.83 ± 6.78

2.

W63A

*

*

*

3.

Y128A

*

*

*

4.

W144A

*

*

*

5.

Y124A

23.92 ± 0.10

823.58 ± 3.12

34.81 ± 0.19

6.

W187A

140.36 ± 1.15

4258.08 ± 89.73

28.75 ± 0.61

7.

W187A_Y124A

45.75 ± 0.23

1080.65 ± 23.65

23.62 ± 0.26

  1. *not performed. All values are expressed as mean ± SEM, based on three individual experiments