Table 3 Physical properties of rBAxn and engineered enzymes using 1% beechwood xylan and 2% CMC as a substrate. – Activity not detected
Enzymes | Xylan | CMC | ||
|---|---|---|---|---|
pH | Temperature | pH | Temperature | |
rBAxn | 9 | 50 | – | – |
W63A | 8 | 50 | 5 | 50 |
Y128A | 5 | 50 | 5 | 50 |
W144A | 7 | 50 | 5 | 50 |
Y124A | 8 | 50 | 5 | 50 |
W187A | 8 | 50 | 8 | 50 |
W187A_Y124A | 7 | 50 | 5 | 50 |