Effects of substrate binding site residue substitutions of xynA from Bacillus amyloliquefaciens on substrate specificity

Table 2 Specific activities of engineered and non-engineered enzymes using 1% beechwood xylan, 2% CMC, 2% Avicel and Whatman filter paper as substrate. – activity not detected

Sr. No.	Enzyme	Relative Specific Activity (%)^a	Specific activity (IU/mg)
Sr. No.	Enzyme	Xylan	CMC	Avicel	FP
1.	rBAxn	100	–	–	–
2.	W63A	7.5	0.139 ± 0.0017	–	–
3.	Y128A	8.02	0.214 ± 0.0023	–	–
4.	W144A	5.4	0.210 ± 0.0015	–	–
5.	Y124A	40.7	0.140 ± 0.0012	–	–
6.	W187A	44.2	0.247 ± 0.002	–	–
7.	W187A_Y124A	19.7	0.289 ± 0.025	11.23 ± 0.029	1.97 ± 0.023

^aThe specific activities (in IU/mg) of rBAxn on xylan is 80.12 ± 2.52. All values are expressed as mean ± SEM, based on three individual experiments

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