Properties | Proteins | ||||
---|---|---|---|---|---|
iYTnC2 | NTnC | ||||
apo | sat | apo | sat | ||
Absorbance maximum (nm) | 499 (413) | 410 | 505 | ||
Emission maximum (nm) | 518 (516) | 520 | 518 | ||
Quantum yielda | 0.33 ± 0.03 (0.041 ± 0.005) | 0.033 ± 0.004 | 0.71 ± 0.05 | 0.65 ± 0.04 | |
ε (mM−1 cm− 1) | 37 ± 1 (14.8 ± 0.4)c | 31.6 ± 0.7b | 108 ± 6b | 52 ± 1c | |
Brightness (%) | 16 (0.8) | 1.4 | 100 | 44 | |
Fluorescence contrast (fold) | 0 mM Mg2+ | 8.1 ± 0.4 | 2.0 ± 0.3 | ||
1 mM Mg2+ | 5.5 ± 0.2 | 2.0 ± 0.7 | |||
pKa | 7.4 ± 0.1 | 8.5 ± 0.1 | 6.09 ± 0.07 | 6.08 ± 0.02 | |
Kd (nM)d | 0 mM Mg2+ | 295 ± 9 [n = 1.7 ± 0.1] | 84 ± 6 [n = 1.9 ± 0.1] | ||
1 mM Mg2+ | 331 ± 22 [n = 1.6 ± 0.2] | 192 ± 40 [n = 2.0 ± 0.4] | |||
Kdkin (nM)e | 200 ± 150 [n = 1.9 ± 0.1] | 94 ± 9 [n = 2.3 ± 0.1] | |||
kon (s−1× M-n)f | 6.2 ± 0.5 × 1012 | 6 × 1015 | |||
koff (s−1)g | 1.12 ± 0.01 | 0.8 ± 0.1; 0.05 ± 0.01h | |||
t1/2off (s)g | 0.58 ± 0.01 | 3.00 ± 0.05 | |||
Maturation half-time (min)i | 3 | ND | 23 | 28 | |
Photobleaching half-time (s)j | 49 ± 19 | ND | 40 ± 8 | 70 ± 5 |