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Fig. 4 | BMC Biotechnology

Fig. 4

From: Genetically encoded calcium indicator with NTnC-like design and enhanced fluorescence contrast and kinetics

Fig. 4

Response of iYTnC and iYTnC2 to Ca2+ variations as a result of spontaneous activity in cultured neurons. a Dissociated neuronal culture co-expressing iYTnC (a, left) and R-GECO1 (a, right) calcium indicators. b - d The graphs illustrate changes in red fluorescence of R-GECO1 (excitation 561 nm) and green fluorescence of iYTnC (b), NTnC (c) or iYTnC2 (d) (excitation 488 nm) as a result of spontaneous activity in neuronal culture. The graph on panel b illustrates changes in fluorescence in the area indicated with white circle in panel a. The minimal fluorescence values were normalized to the unit

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