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Fig. 3 | BMC Biotechnology

Fig. 3

From: Genetically encoded calcium indicator with NTnC-like design and enhanced fluorescence contrast and kinetics

Fig. 3

Response of the iYTnC and iYTnC2 indicators to Ca2+ concentration changes in HeLa cells. a Confocal images of HeLa Kyoto cells co-expressing green iYTnC (a, left) and red R-GECO1 (a, right) calcium indicators. b-d The graphs illustrate changes in green fluorescence of iYTnC (b), NTnC (c) or iYTnC2 (d) indicators and in red fluorescence of the reference co-expressed R-GECO1 GECI in response to addition of 2 mM CaCl2 and 2.5 μM ionomycin. The changes in panel b correspond to the area indicated with white circles in the panel a

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BMC Biotechnology

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