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Fig. 2 | BMC Biotechnology

Fig. 2

From: Genetically encoded calcium indicator with NTnC-like design and enhanced fluorescence contrast and kinetics

Fig. 2

Calcium association and dissociation kinetics for the iYTnC2 and GCaMP6f indicators studied using stopped-flow fluorimetry. a Calcium association kinetics curves for iYTnC2. b Observed Ca2+ association rate constants determined from association curves for iYTnC2 and control GCaMP6f GECIs. For the iYTnC2 indicator, fast (green) and slow (grey) exponents are shown. c Relative contribution of monoexponents A1/(A1 + A2) and A2/(A1 + A2) for the iYTnC2 indicator, where A1 and A2 are pre-exponential factors in the association curve equation ΔFlu(t) = A1*exp.(-Kobs1*t)-A2*exp.(-Kobs2*t). d Calcium dissociation kinetics for the iYTnC2, NTnC and GCaMP6f GECIs. Starting concentration of Ca2+ was 1000 nM

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