Skip to main content

Table 3 Information on the insertion sites and Luc expression of the different transformants. Five low and five high Luc expressors were collected and analyzed for the characterization of the insertion site both for Agrobacterium transformation and electroporation. The first column reports the name of the transformant selected (see Additional file 7: Table S4). Luc activity of the transformants at the 3rd and 20th subculture in selective medium is expressed as average values of CPS (counts per second) normalized for 105 cells ± standard deviation of three biological replicates. Chr #: chromosome number in which DNA integration occurred. Insertion points were determined from the Chlamydomonas reinhardtii structural annotation v5.5 [32]. Gene annotation was taken from the Chlamydomonas reinhardtii functional annotation v 5.5 [32]. Gene expression refers to RNA-Seq experiments conducted on wild type cells grown in TAP medium [32, 36] and are reported in FPKM (fragments per kilobase per million mapped fragments)

From: Agrobacterium-mediated and electroporation-mediated transformation of Chlamydomonas reinhardtii: a comparative study

Transformant Luc activity 3rd subculture Luc activity 20th subculture Chr # Insertion point Gene name Gene annotation Gene expression (FPKM)
CPS ± sd CPS ± sd
Transformation method: Agrobacterium
 B 20 9 ± 2 8 ± 1 8 Gene (3′ UTR) Cre08.g381050 Senescence-associated gene 12 4.65
 A 14 38 ± 11 18 ± 2 1 Gene (exon) Cre01.g051900 Ubiquinol-cytochrome C reductase iron-sulfur subunit 166.65
 B 9 5 ± 1 64 ± 12 12 Gene (5′UTR) Cre12.g486000 n.a. 1.49
 B 12 7 ± 1 69 ± 13 17 Gene (exon) Cre17.g736700 n.a. 0
 A 18 48 ± 10 16 ± 2 1 Gene (exon) Cre01.g015250 DNA binding; DNA-directed DNA polymerases 4.13
 A 6 13,306 ± 1218 6979 ± 926 3 Gene (exon) Cre03.g204200 n.a. 0
 A 7 19,240 ± 1945 21,210 ± 1694 n.d. n.d. n.d. n.d. n.d.
 B 4 15,202 ± 2795 8006 ± 1259 9 Gene (5′UTR) Cre09.g398067 Rotamase FKBP 1 9.45
 B 6 12,460 ± 1239 7751 ± 643 17 Gene (exon) Cre17.g699600 Cre08.g377150 Sedoheptulose-bisphosphatase 14.62
    8 Gene (exon) n.a. 18.19
 B 11 16,458 ± 892 8762 ± 831 8 Gene (3′ UTR) Cre08.g379400 n.a. 2.95
Transformation method: Electroporation
 A 2 7 ± 3 49 ± 6 12 Gene (3′ UTR) Cre12.g560450 n.a. 3.08
 A 3 2 ± 0 68 ± 9 1 Gene (exon) Cre01.g014150 MATE efflux family protein 5.20
4 Gene (exon) Cre04.g223550 n.a. 0.42
 A 4 4 ± 1 24 ± 5 13 Gene: 3′ UTR Cre13.g569850 Ammonium transporter 1;2 0.89
 A 5 37 ± 11 61 ± 7 12 Gene (exon) Cre12.g559450 Calcium-dependent lipid-binding family protein 37.98
 A 7 1 ± 1 21 ± 5 11 Gene (exon) Cre11.g467556 n.a. 2.73
5 Gene (5′UTR) Cre05.g232100 n.a. 6.87
 A 10 17,849 ± 1022 16,393 ± 3650 3 Gene (3′ UTR) Cre03.g186050 n.a. 1.26
 A 11 11,347 ± 645 3932 ± 771 2 2 Genes, both 3′UTR Cre02.g098700; ABC-2 type transporter family protein 0.63
  Intergenic region Cre02.g098750 STELAR K+ outward rectifier 2.77
8   /   /
 B 11 17,253 ± 1316 20,099 ± 2464 3 Gene (intron) Cre03.g165700 Pyruvate decarboxylase-2 87.52
 A 18 13,348 ± 753 15,958 ± 1918 3 Intergenic region / / /
 B 18 12,482 ± 499 13,977 ± 1959 16 Intergenic region / / /
  1. n.d. not determined, n.a. not available