Fig. 3

T-DNA deletion pattern in pAgroLucR and pAgroLucL transformants. The pAgroLucR and pAgroLucL plasmids were used for Agrobacterium mediated transformation and integrity of the T-DNA in the resulting transformants was tested via PCR with appropriate oligonucleotide pairs. Panels a (pAgroLucR) and b (pAgroLucL) show the position of the amplicons (top in each box) and the frequency of transformants showing amplification for each amplicon (bottom in each box). A set of 29 independent transformants were tested for each construct. All transformants contain amplicon 1, corresponding to the Paro gene. When Luc is cloned next to the LB is retained at higher frequency (pAgroLucL, amplicons 2 and 5 in Panel b) with respect to the RB (pAgroLucR, amplicons 2 and 4). See also Additional file 5: Figure S5 and Additional file 6: Figure S6. Oligonucleotide sequences are reported in Additional file 7: Table S3