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Fig. 2 | BMC Biotechnology

Fig. 2

From: Agrobacterium-mediated and electroporation-mediated transformation of Chlamydomonas reinhardtii: a comparative study

Fig. 2

Deletions along the T-DNA in transformants obtained with electroporation or Agrobacterium-mediated transformation. Chlamydomonas cw15 cells were transformed with the pAgroLucR plasmid, using Agrobacterium or electroporation. Integrity of the inserted T-DNA was tested by PCR amplification with nested pairs of oligonucleotides spanning the T-DNA (Panel a) in a set of nine independent transformants. The histogram (Panel b) shows that there is a gradient of deletion from the LB (Amplicon1) to the RB (Amplicon 6) independently of the transformation method used (Agrobacterium, black; electroporation, gray). Oligonucleotide sequences are reported in Additional file 7: Table S2

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