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Fig. 1 | BMC Biotechnology

Fig. 1

From: Agrobacterium-mediated and electroporation-mediated transformation of Chlamydomonas reinhardtii: a comparative study

Fig. 1

Schematic map of the vectors used for Agrobacterium-mediated transformation. Only the portion between the LB and RB (T-DNA) is shown. All vectors contain the Paro gene, conferring resistance to paromomycin under the control of the strong HSP70/RBCS2 hybrid promoter (P1) and RBCS2 terminator (T1). pAgroR (a) contains an expression cassette containing the PSAD promoter (P2) and terminator (T2) sequences. In pAgroLucR (b) the Luc coding sequence is cloned in the expression cassette. In pAgroLucL (c) the Paro and Luc cassettes have an inverted orientation with respect to pAgroLucR. For further details see Material and Methods

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BMC Biotechnology

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