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Table 2 RNase If -qPCR assay inter-assay precision

From: RNase If -treated quantitative PCR for dsRNA quantitation of RNAi trait in genetically modified crops

  dsRNA input for qPCR (pg) Run 1 Cp Run 2 Cp Run 3 Cp CV of CP Average of CV
STD1 12.07 14.64 14.02 13.38 4.5% 5.0%
STD2 4.02 16.35 16.08 15.17 3.9%  
STD3 2.01 17.66 17.42 16.16 4.7%  
STD4 1.01 19.06 18.76 17.03 6.0%  
STD 5 0.50 20.52 20.13 18.67 4.9%  
STD6 0.25 21.82 21.68 19.95 4.9%  
STD7 0.13 22.59 22.07 19.79 6.9%  
STD8 0.06 24.57 24.55 23.03 3.7%  
STD9 0.03 24.71 24.77 22.26 6.0%  
STD 10 0.02 25.80 25.76 23.82 4.5%  
  1. 10-point standard synthetic v-ATPase C dsRNA was assayed in there independent runs by different analysts using the standard protocol described in Fig. 1. dsRNA amount (pg) in a 10 μL qPCR reaction and the corresponding mean (N = 3) Cp (crossing point-PCR-cycle) from each individual run are presented. The correlation between Cp and Log 2 RNA amounts are presented here. The coefficient of variation (CV) was calculated using the Cp values of each standard point