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Fig. 5 | BMC Biotechnology

Fig. 5

From: RNase If -treated quantitative PCR for dsRNA quantitation of RNAi trait in genetically modified crops

Fig. 5

Investigation of dosage effect using RNase If -qPCR. The total RNAs isolated from distinct transgenic maize events from a segregating S1 population (including homozygous and hemizygous plants) of different constructs (listed on top) carrying v-ATPase C. The RNA were analyzed using the RNaseIf - qPCR assay. The transcripts level were analyzed by 95 °C (RNase If), and 70 °C (without RNase If treatment) to quantify v-ATPase C dsRNA, and the endogenous TIP41 gene, respectively. The v-ATPase C dsRNA RTL for each biological replicate was normalized to endogenous gene (TIP41). The mean RTL (N = 12) are denoted in the box plot

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