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Fig. 3 | BMC Biotechnology

Fig. 3

From: RNase If -treated quantitative PCR for dsRNA quantitation of RNAi trait in genetically modified crops

Fig. 3

RNase If -qPCR assay linearity. 10-point standard of synthetic dsRNAs and sRNAs were analyzed using RNase If -qPCR described in Fig. 1. The synthetic RNAs were analyzed by 95 °C (RNase If), and 70 °C (without RNase If), to quantify dsRNAs (a), and ssRNAs (b), respectively. The relative transcript level (RTL) was obtained via normalization of target signal over the endogenous gene (TIP41). The correlation between log2 of RTL and RNA amounts are presented here. The equation of trend line and R2 is shown

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