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Fig. 2 | BMC Biotechnology

Fig. 2

From: RNase If -treated quantitative PCR for dsRNA quantitation of RNAi trait in genetically modified crops

Fig. 2

Northern blot to characterize the synthetic ssRNA and dsRNA. a RNAi targeted to Dv v-ATPase C transgene design cassette scheme. The transgene is comprised of a promoter and terminator between which an inversely-repeated sequence of the target gene is inserted with a spacer region between the repeats (promoter and terminator informaiton are described in Methods). Synthetic dsRNA and ssRNA are shown as diagram here. The thick grey line indicates the antisense RNA probe used for the RNA blot. The black arrows denote the primer location used in qRT-PCR. b Synthetic dsRNA and ssRNA (spiked into wild-type maize leaf B104 RNA) were treated with RNase If. The non-treated RNA were used as control. The RNA blot was probed with the 149 bp DIG-labeled antisense RNA probe (shown in gray bar in A). The arrow on the right indicated the approximate molecular size

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