Fig. 4

Localization efficiency variation across cell line and seeding density. Localization efficiency was calculated as the percent of cells in microwells relative to total cells on the substrate after removal of the stencil film. Four hundred random wells with surrounding area were sampled from each array. No data were collected for K562 cells in the No Stencil condition, because few or no K562 cells adhered to the substrate without the stencil. Data were pooled from four independent experiments. Error bars indicate SEM