Fig. 3

Cell seeding localization efficiency. a Composite fluorescent (nuclei, blue) and phase contrast (microwells) micrographs of K562, HME1, and MDA-MB-231 cells seeded with (top) and without (bottom) a polymer stencil. Cells were imaged after cell adhesion and removal of the stencil film. b Mean and standard error of localization efficiency for substrates seeded at 100 K cells/mL with and without a polymer stencil. Exclamation point (!) indicates a lack of reliable data due to cells being non-adherent in the absence of the stencil. Data were pooled from four independent experiments. P-values determined by Mann-Whitney test