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Table 3 Catalytic activity and regioselectivity of TLL immobilized on Purolite C18 under different experimental conditions

From: Modulation of the regioselectivity of Thermomyces lanuginosus lipase via biocatalyst engineering for the Ethanolysis of oil in fully anhydrous medium

Immobilization conditions

(pH; presence/absence CTAB; Tª)

Ethanolysis of oil: μmols/min/

gram of biocatalyst

Regioselectivity

pH 5; no CTAB; 4 °C

0.5

No selectivity

pH 7, no CTAB; 25 °C

0.6

No selectivity

pH 7; with CTAB; 25 °C

0.8

No selectivity

pH 8.5; no CTAB; 30 °C

0.8

No selectivity

pH 8.5; with CTAB; 30 °C

2.5

1,3-regioselectivity