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Fig. 7 | BMC Biotechnology

Fig. 7

From: Modulation of the regioselectivity of Thermomyces lanuginosus lipase via biocatalyst engineering for the Ethanolysis of oil in fully anhydrous medium

Fig. 7

Effect of the Sepabeads C18 in the acyl migration. Symbols: FAEE production by TLL immobilized on Sepabeads C18 (dash line and filled circle); 2-MAG concentration in the presence of active TLL immobilized on Sepabeads C18 (unfilled circle); 2-MAG concentration in the presence of inactivated Sepabeads C18 TLL derivative (filled triangle); and 2-MAG concentration in the presence of Sepabeads C18 without TLL (unfilled triangle). Ethanolysis was carried out using 0.1 g of fully dried support with (20 mg TLL/g support) or without TLL. A volume of 0.6 mL of anhydrous ethanol (5.2 mM) and 1.2 mL of 2-monoolein (0.65 mM) were added in order to start the ethanolysis. The reaction was carried out at 40 °C and the reaction suspension was stirred in an orbital shaker at 150 rpm. 0.65 mM was considered as 100%. The course of the reaction was analyzed by RP-HPLC

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BMC Biotechnology

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