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Fig. 6 | BMC Biotechnology

Fig. 6

From: Modulation of the regioselectivity of Thermomyces lanuginosus lipase via biocatalyst engineering for the Ethanolysis of oil in fully anhydrous medium

Fig. 6

Time course of ethanolysis reaction of HOS- oil performed with TLL immobilized on Purolite C18 at pH 8.5, 30 °C with CTAB (a) and at pH 5.0, 4 °C without CTAB (b). Symbols: FAEE (filled circle); TG (unfilled circle); 2-MAG (filled square); DG (filled triangle). Ethanolysis was carried out using 0.1 g of fully dried derivative (20 mg TLL/g support). A volume of 0.6 mL of anhydrous ethanol (5.2 mM) and 1.2 mL of sunflower oil (0.65 mM) were added in order to start the ethanolysis reaction. The reaction was carried out at 40 °C and the reaction suspension was stirred in an orbital shaker at 150 rpm. The course of the reaction was analyzed by RP-HPLC

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BMC Biotechnology

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