Fig. 3From: Heterologous expression, purification and function of the extracellular domain of human RANKBiacore T100 analysis of RANKL/RANK-N binding. Concentrations of RANK-N (from 32.5 to 1000 nM) were passed over a CM5 chip that was coated with RANKL. The data are plotted as the steady-state response (in RU) vs RANK-N concentration and fit to a rearranged Hill equation, resulting in a KD of 2.62×10−6 MBack to article page