Fig. 2
From: Heterologous expression, purification and function of the extracellular domain of human RANK
Purification of RANK-N and glycosylation analysis. a The growth curve of Pichia pastoris in a shake flask at 30 °C. b SDS-PAGE analysis of purified RANK-N protein. M: molecular weight marker; Lane 1: Induction by methanol at 96 h; Lane 2: Flow-through of ultrafiltration; Lane 3: Sample after ultrafiltration; Lane 4: Fraction 1 of Sephadex G-50 gel chromatography; Lane 5: Flow-through from Q-Sepharose-FF chromatography; lanes 6–9: Fraction 1 of Q-Sepharose Fast Flow chromatography. c Treatment of the protein with PNGase F. Lane 1: molecular weight marker; Lane 2: RANK-N; Lane 3: RANK-N+ PNGase F, arrow indicates PNGase F