Fig. 1
From: Heterologous expression, purification and function of the extracellular domain of human RANK
Expression and characterization of recombinant RANK-N. a Samples were taken from culture medium of pPIC9K /RANK-N after 0, 12, 24, 36, 48, 60, 72, 84 and 96 h of induction by methanol. The supernatant samples were separated in a 12% (w/v) SDS-PAGE gel and stained with Coomassie Brilliant Blue R-250. b Western blot analysis of RANK-N expression after 0, 12, 24, 36, 48, 60, 72, 84 and 96 h of induction by methanol