Fig. 3
From: Light induced expression of β-glucosidase in Escherichia coli with autolysis of cell
Bgl1A(A24S/F297Y) activity and SDS-PAGE assay of extracellular and intracellular fraction after cell autolysis in flasks. Slash (extracellular) and gray (intracellular) reflect β-glucosidase activity when SRRz lysis gene was induced with IPTG, vertical (extracellular) and dark gray (intracellular) reflect enzyme activity when autolysis was induced with lactose (a). The soluble and insoluble fraction of cell lysate was assayed by SDS-PAGE when autolysis was induced with lactose (b), M is molecular marker, lysis is supernatant of fermentation culture after cell autolysis, super and precip are supernatant and precipitate after ultrasonic fragmentation of cells that were not autolysed