Fig. 5

Scheme of inoculation and serum obtention. a Mice were immunized at Day 1, a booster dose was given at Day 23, a serum sample was taken at Day 30, an additional booster dose of incomplete Freund’s adjuvant (IFA) emulsion was given at Day 40 by employing extract nuclei expressing rSN1; and total serum was collected at Day 53. b Western blot analysis of extract nuclei of Sf9 cells either expressing rSN1 (+) or not (−). M: PageRuler Prestained Protein Ladder. Three different antibodies were used to reveal commercial anti-HIS, serum sample and total polyclonal serum (15% SDS-PAGE). The arrow indicates the rSN1 peptide