Skip to main content
Fig. 4 | BMC Biotechnology

Fig. 4

From: Successful production of the potato antimicrobial peptide Snakin-1 in baculovirus-infected insect cells and development of specific antibodies

Fig. 4

Purification steps of the rSN1 fusion protein. Upper panel: Western blot analysis using an anti-HIS antibody. Lane 1: Total nuclear lysate, Lane 2: Unbound proteins flowed through the column. Lanes 3–4: Proteins eluted from wash buffers of different stringent conditions tested (20 mM or 30 mM imidazole respectively). Lanes 5–6: Proteins eluted from acidic buffers of different pH 5.9 (lane 5) or pH 4.5 (lane 6). Lane 7: Proteins eluted (up to 6.3 mg/ml) with high concentrations of imidazole (500 mM). Lower panel: The nitrocellulose membrane stained with Ponceau dye for protein detection after the 10% SDS-PAGE analysis of representative purification steps of the rSN1. M: BenchMark Pre-Stained Protein Ladder. The arrow indicates the rSN1 peptide

Back to article page