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Table 3 Expression levels of P0 and P1mediated expression

From: A new single-step protocol for rapid baculovirus-driven protein production in insect cells

Construct cells Temp [°C] % of TCP* P0 % of TCP* P1
for 72 h    
#1 Hi5 26C 31.6 45.9
#1 Hi5 20C 37.9 53.7
#1 Sf9 26C 3.5 2.5
#1 Sf9 20C nd 6.1
#2 Hi5 26C 43.7 32.3
#2 Hi5 20C 34.5 34.4
#2 Sf9 26C 10.5 nd
#2 Sf9 20C 26.6 nd
#3 Hi5 26C 52.4 51.9
#3 Hi5 20C 58.7 38.4
#3 Sf9 26C 19.1 3.3
#3 Sf9 20C 12.5 7.2
#4 Hi5 26C 17.5 26
#4 Hi5 20C 23.6 26.2
#4 Sf9 26C 5.9 4
#4 Sf9 20C 8.5 7.7
for 96 h    
#1 Hi5 26C 44.9 31
#1 Hi5 20C 46.6 46.7
#2 Hi5 26C 31.5 27.9
#2 Hi5 20C 40.1 36.9
#3 Hi5 26C 54.1 47.7
#3 Hi5 20C 56.7 61.1
#4 Hi5 26C 19 24.5
#4 Hi5 20C 28.1 26.8
  1. Expression levels [%] of *total cellular protein derived from Cy5 quantification of P0 versus P1 total cell lysates of construct #1, #2, #3, #4 expressed in Sf9 and Hi5 at 20 °C and 26 °C for 72 h and in Hi5 for 96 h