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Table 3 Expression levels of P0 and P1mediated expression

From: A new single-step protocol for rapid baculovirus-driven protein production in insect cells

Construct

cells

Temp [°C]

% of TCP* P0

% of TCP* P1

for

72 h

   

#1

Hi5

26C

31.6

45.9

#1

Hi5

20C

37.9

53.7

#1

Sf9

26C

3.5

2.5

#1

Sf9

20C

nd

6.1

#2

Hi5

26C

43.7

32.3

#2

Hi5

20C

34.5

34.4

#2

Sf9

26C

10.5

nd

#2

Sf9

20C

26.6

nd

#3

Hi5

26C

52.4

51.9

#3

Hi5

20C

58.7

38.4

#3

Sf9

26C

19.1

3.3

#3

Sf9

20C

12.5

7.2

#4

Hi5

26C

17.5

26

#4

Hi5

20C

23.6

26.2

#4

Sf9

26C

5.9

4

#4

Sf9

20C

8.5

7.7

for

96 h

   

#1

Hi5

26C

44.9

31

#1

Hi5

20C

46.6

46.7

#2

Hi5

26C

31.5

27.9

#2

Hi5

20C

40.1

36.9

#3

Hi5

26C

54.1

47.7

#3

Hi5

20C

56.7

61.1

#4

Hi5

26C

19

24.5

#4

Hi5

20C

28.1

26.8

  1. Expression levels [%] of *total cellular protein derived from Cy5 quantification of P0 versus P1 total cell lysates of construct #1, #2, #3, #4 expressed in Sf9 and Hi5 at 20 °C and 26 °C for 72 h and in Hi5 for 96 h