Fig. 3

a P0 versus P1 total cell lysates of construct #1 expressed in Sf9 and Hi5 cells at 20 °C and 26 °C for 72 h visualized by Cy5 labelling of Easy SDS-PAGE (b) P0 versus P1 total cell lysates of construct #1, #2, #3, #4 expressed in Hi5 cells at 20 °C and 26 °C for 96 h visualized by Cy5 labelling and Easy SDS-PAGE; expression levels of total cellular protein [%] for (A) and (B) are depicted in Table 3; (c) P0 versus P1 culture supernatant of construct #5 and #6 expressed in Sf9 (S) and Hi5 (H) cells at 20 °C and 26 °C for 72 h or 96 h, loaded on 4–12% Bis-Tris Nu-PAGE and visualized by Western Blotting using HisProbe™-HRP