Generation of murine macrophage-derived cell lines expressing porcine CD163 that support porcine reproductive and respiratory syndrome virus infection

Table 1 Primer list for full-length of genome amplification or cytokines expression

Genes	Forward primer	Reverse primer	References
CD163	GCTCTAGAATGGTGCTACTTGAAG	CGGGATCCTCATTGTACTTCAGAGTGG	Xiangpeng wang et al. 2013
ORF7	ATGCCAAATAACAACGGCAAGCAGC	TCATGCTGAGGGTGATGCTGTG	Xiangpeng wang et al. 2013
GAPDH	CCTTCCGTGTCCCTACTGCCAAC	GACGCCTGCTTCACCACCTTCT	Xin-xin Chen et al. 2014
TNFα	^a AACCTCAGATAAGCCCGTCG ^b GGCAGGTCTACTTTGGAGTCAT	ACCACCAGCTGGTTGTCTTT CAGAGTAAAGGGGTCAGAGTGG	Gudmundsdottir and Risatti 2009 Tingyu Wang et al. 2014
IL4	^a GCCGGGCCTCGACTGT ^b CATCGGCATTTTGAACGAG	TCCGCTCAGGAGGCTCTTC TGGAAGCCCTACAGACAAGC	Dawson et al. 2005
IL10	^a CGGCGCTGTCATCAATTTCTG ^b GGACAACATACTGCTAACCGACT	CCCCTCTCTTGGAGCTTGCTA TGGGGCATCACTTCTACCA	Duvigneau et al. 2005
IFNγ	^aAATGGTAGCTCTGGGAAACTG ^b TGC TGA TGG GAG GAG ATGTCT	ACTTCTCTTCCGCTTTCTTAGG TGC TGT CTG GCC TGC TGT TA	Yoo Jin Lee et al. 2012; Yinhang Yu et al.2016
TGFβ	^a CGCCTGCTGAGGCAAAGT ^b TGACGTCACTGGAGTTGTACGG	GAGGTAGCGCCAGGAATCATT GGTTCATGTCATGGATGGTGC	Min Song et al. 2011

IL interleukin, TNF tumor necrosis factor, TGF transforming growth factor; ^aprimers of swine-origin; ^bprimers of mouse-origin