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Table 2 Box-Behnken (BB) screening design

From: Optimization of physical conditions for the production of thermostable T1 lipase in Pichia guilliermondii strain SO using response surface methodology

RUN Factor: 1
A:pH
Factor: 2
B: IT (h)
Factor: 3
C: IS (%)
LA (U/mL) OD600 (nm)
1 10 36 4 1.8 24.9
2 10 48 3 0.9 24.4
3 8 36 3 1.2 21.8
4 6 36 2 1.6 22.3
5 8 48 2 0.9 23.9
6 6 48 3 1.0 21.0
7 8 36 3 1.1 23.6
8 8 24 2 1.9 24.6
9 8 48 4 0.7 24.4
10 8 36 3 0.9 26.0
11 6 36 4 1.4 21.4
12 10 24 3 2.1 22.6
13 8 36 3 0.6 21.3
14 8 24 4 1.6 22.8
15 6 24 3 1.8 21.9
16 10 36 2 2.7 22.1
17 8 36 3 1.6 22.3
  1. Run-flask number, IS inoculum size, IT incubation time, LA lipase activity, OD optical density