Skip to main content

Table 2 Box-Behnken (BB) screening design

From: Optimization of physical conditions for the production of thermostable T1 lipase in Pichia guilliermondii strain SO using response surface methodology

RUN

Factor: 1

A:pH

Factor: 2

B: IT (h)

Factor: 3

C: IS (%)

LA (U/mL)

OD600 (nm)

1

10

36

4

1.8

24.9

2

10

48

3

0.9

24.4

3

8

36

3

1.2

21.8

4

6

36

2

1.6

22.3

5

8

48

2

0.9

23.9

6

6

48

3

1.0

21.0

7

8

36

3

1.1

23.6

8

8

24

2

1.9

24.6

9

8

48

4

0.7

24.4

10

8

36

3

0.9

26.0

11

6

36

4

1.4

21.4

12

10

24

3

2.1

22.6

13

8

36

3

0.6

21.3

14

8

24

4

1.6

22.8

15

6

24

3

1.8

21.9

16

10

36

2

2.7

22.1

17

8

36

3

1.6

22.3

  1. Run-flask number, IS inoculum size, IT incubation time, LA lipase activity, OD optical density