Skip to main content

Table 1 Plackett-Burman screening Design Methodology

From: Optimization of physical conditions for the production of thermostable T1 lipase in Pichia guilliermondii strain SO using response surface methodology

Run Factor 1
A: T (°C)
Factor 2
B: pH
Factor 3
C: IS (%)
Factor 4
D: IT (h)
Factor 5
E: AS (rpm)
Factor 6
F: CV (mL)
Response 1
LA (U/mL)
Response 2
OD600 (nm)
1 30.00 6.00 2.00 24.00 300.00 50.00 1.4 16. 5
2 30.00 10.00 2.00 24.00 250.00 200.00 2.5 24.8
3 20.00 6.00 4.00 24.00 300.00 200.00 1.0 17.9
4 20.00 6.00 2.00 48.00 250.00 200.00 1.5 27.9
5 20.00 10.00 4.00 48.00 250.00 50.00 1.3 20.9
6 20.00 10.00 2.00 48.00 300.00 50.00 2.4 23.9
7 20.00 10.00 4.00 24.00 300.00 200.00 2.3 20.2
8 20.00 6.00 2.00 24.00 250.00 50.00 1.5 25.6
9 30.00 10.00 2.00 48.00 300.00 200.00 1.9 21.8
10 30.00 6.00 4.00 48.00 250.00 200.00 1.0 22.0
11 30.00 10.00 4.00 24.00 250.00 50.00 2.4 25.4
12 30.00 6.00 4.00 48.00 300.00 50.00 1.4 22.3
  1. LA lipase activity, Factors same as parameters, Response same as observation and OD-Optical Density