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Table 1 Plackett-Burman screening Design Methodology

From: Optimization of physical conditions for the production of thermostable T1 lipase in Pichia guilliermondii strain SO using response surface methodology

Run

Factor 1

A: T (°C)

Factor 2

B: pH

Factor 3

C: IS (%)

Factor 4

D: IT (h)

Factor 5

E: AS (rpm)

Factor 6

F: CV (mL)

Response 1

LA (U/mL)

Response 2

OD600 (nm)

1

30.00

6.00

2.00

24.00

300.00

50.00

1.4

16. 5

2

30.00

10.00

2.00

24.00

250.00

200.00

2.5

24.8

3

20.00

6.00

4.00

24.00

300.00

200.00

1.0

17.9

4

20.00

6.00

2.00

48.00

250.00

200.00

1.5

27.9

5

20.00

10.00

4.00

48.00

250.00

50.00

1.3

20.9

6

20.00

10.00

2.00

48.00

300.00

50.00

2.4

23.9

7

20.00

10.00

4.00

24.00

300.00

200.00

2.3

20.2

8

20.00

6.00

2.00

24.00

250.00

50.00

1.5

25.6

9

30.00

10.00

2.00

48.00

300.00

200.00

1.9

21.8

10

30.00

6.00

4.00

48.00

250.00

200.00

1.0

22.0

11

30.00

10.00

4.00

24.00

250.00

50.00

2.4

25.4

12

30.00

6.00

4.00

48.00

300.00

50.00

1.4

22.3

  1. LA lipase activity, Factors same as parameters, Response same as observation and OD-Optical Density