Skip to main content
Fig. 4 | BMC Biotechnology

Fig. 4

From: Production and purification of chimeric HBc virus-like particles carrying influenza virus LAH domain as vaccine candidates

Fig. 4

Final purification (a) and characterization (b-e) of LAH3-HBc VLPs. a Fractogel TMAE chromatography profile. VLP and free nucleic acid (NA) peaks are indicated. b Coomassie-stained PAAG of final product after dialysis and concentration. 1, 2 and 4 μl of protein are loaded per track. c Western blotting of final product. Lane 1, negative control; lane 2, K1-K1 VLPs; lane 3, LAH3-HBc VLPs. Full-length protein and product related bands are marked by arrowhead and asterix, respectively. d Nucleic acid content of final product in native agarose gel. Visible is only VLP-associated NA band (indicated by arrowhead). e Quality of LAH3-HBc VLPs verified by electron microscopy

Back to article page

BMC Biotechnology

ISSN: 1472-6750

Contact us