Fig. 3
From: AFEAP cloning: a precise and efficient method for large DNA sequence assembly
Assembly efficiency of multiple DNA fragments with AFEAP cloning. a Schematic details show the mechanism for the number of fragments characterization. The DNA sequence encoding T4 L and MCM6 proteins is split up into a number of fragments as shown by double-headed arrows. The number of fragments for each assembly is shown on the left of the double-headed arrows and the join sites for assembly is indicated on the below of dash lines. T4 L: T4 lysozyme. V: vector (backbone). b Effects of molar ratio between longer and shorter DNA fragments. c CFU and fidelity as a function of fragment number. d Effects of transformation conditions