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Fig. 2 | BMC Biotechnology

Fig. 2

From: AFEAP cloning: a precise and efficient method for large DNA sequence assembly

Fig. 2

Determination of parameters for effective assembly. a Flowsheet of the assembly of 3′ and 5′ ends of linear DNA fragment to form the circle. Effects of the overhang length on the assembly efficiency were characterized as colony-forming units (CFUs) per microgram of ligated DNA (b) and percent of colonies correct (fidelity) (c). d Effects of the overhang designed as 5′ end of G/C or A/T. The relative CFUs produced of overhang designed as 5′ end of G or C are presented as percentages of the CFUs of overhang designed as 5′ end of A or T at the same construct size. e Effects of the ligase treated. The relative CFUs produced of ligase treated are presented as percentages of the CFUs of no ligase treated at the same construct size. f Effects of transformation conditions

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