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Fig. 4 | BMC Biotechnology

Fig. 4

From: Adaptation of the GoldenBraid modular cloning system and creation of a toolkit for the expression of heterologous proteins in yeast mitochondria

Fig. 4

Example of the capabilities to generate genome-integrated multiple TU constructs using the GoldenBraid2.0 yeast-adapted system. a Diagram of the construct containing multiple TUs used for the simultaneous expression of nifH, nifM, nifU and nifS. WB analysis of NifH (b), NifM (c) and NifU (d) accumulation in exponentially growing cultures of the W303 strain containing glucose as the carbon source. The lower panel shows the corresponding membrane Coomassie stained as a loading control

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BMC Biotechnology

ISSN: 1472-6750

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