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Table 2 Prediction of yeast growth on SD/−L-W/−H/−A medium in SUT system with different types of proteins

From: A split ubiquitin system to reveal topology and released peptides of membrane proteins

Type

Terminus

NubG

NubWT

Nucleo-cytoplasmic protein

TA-Target

Yesa

Yes

Target-TA

Yesa

Yes

N terminal truncated membrane protein

TA-Target

Yesa

Yes

Target-TA

No

Yesc/Noe

Membrane protein without truncation

TA-Target

No

Yesc/Noe

Target-TA

No

Yesc/Noe

  1. Note. a autoactivation, c cytoplasmic termini, e extracellular termini