Table 1 Strains and plasmid used in this work
Strain | Genotype (description) | Reference |
|---|---|---|
E. coli DH5α | F– Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17 (rK–, mK+) phoA supE44 λ– thi-1 gyrA96 relA1 | Invitrogen |
P. acidipropionici | ATCC4875 | [45] |
JAY270 | Industrial S. cerevisiae PE-2; MATa:MATα | [46] |
LVYA1 | JAY270; MATα; ura3Δ | [40] |
BT | LVYA1; pRS426 | This work |
BTXIPa | LVYA1; pRSXIPa | This work |
BTXI2.0 | LVYA1; pRSXI2.0 | This work |
LVY27 | LVYA1, CEN5::pTDH1-xylA-tTDH1; gre3Δ; CEN2::pADH1-XKS1-tADH1; CEN8::pADH1-XKS1-tADH1; CEN12::pTDH1-TAL1-tTDH1-pPGK1-RKI1-tPGK1; CEN13::pTDH1-TKL1-tTDH1-pPGK1-RPE1-tPGK1 | [40] |
LVY65 | LVY27, xylAΔ, ura3Δ | [40] |
BTY28 | LVY65; pRS426 | This work |
BTY29 | LVY65; pRSXIOrp | This work |
BTY30 | LVY65; pRSXI2.0 | This work |
BTY31 | LVY65; CEN5::pPGK-GroEL-tPGK-URA3-pADH1-GroES-tADH1 | This work |
BTY32 | BTY31; pRS426 | This work |
BTY33 | BTY31; pRSXIOrp | This work |
BTY34 | BTY31; pRSXI2.0; | This work |
Plasmid | ||
pRS426 | ori(f1) - lacZ - T7 promoter - MCS (KpnI-SacI) - T3 promoter - lacI - ori(pMB1) - ampR - ori (2 μm) - ura3 | [47] |
pRSXIPa | pRS426; pTDH1-XIPa-tTDH | This work |
pRSXI2.0 | pRS426; pTDH1-XI2.0-tTDH | This work |
pRSXIOrp | pRS426; pTDH1-XIOrp-tTDH | [40] |