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Table 1 Strains and plasmid used in this work

From: Conversion of an inactive xylose isomerase into a functional enzyme by co-expression of GroEL-GroES chaperonins in Saccharomyces cerevisiae

Strain Genotype (description) Reference
E. coli DH5α F– Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1
hsdR17 (rK–, mK+) phoA supE44 λ– thi-1 gyrA96 relA1
Invitrogen
P. acidipropionici ATCC4875 [45]
JAY270 Industrial S. cerevisiae PE-2; MATa:MATα [46]
LVYA1 JAY270; MATα; ura3Δ [40]
BT LVYA1; pRS426 This work
BTXIPa LVYA1; pRSXIPa This work
BTXI2.0 LVYA1; pRSXI2.0 This work
LVY27 LVYA1, CEN5::pTDH1-xylA-tTDH1; gre3Δ; CEN2::pADH1-XKS1-tADH1; CEN8::pADH1-XKS1-tADH1; CEN12::pTDH1-TAL1-tTDH1-pPGK1-RKI1-tPGK1; CEN13::pTDH1-TKL1-tTDH1-pPGK1-RPE1-tPGK1 [40]
LVY65 LVY27, xylAΔ, ura3Δ [40]
BTY28 LVY65; pRS426 This work
BTY29 LVY65; pRSXIOrp This work
BTY30 LVY65; pRSXI2.0 This work
BTY31 LVY65; CEN5::pPGK-GroEL-tPGK-URA3-pADH1-GroES-tADH1 This work
BTY32 BTY31; pRS426 This work
BTY33 BTY31; pRSXIOrp This work
BTY34 BTY31; pRSXI2.0; This work
Plasmid
 pRS426 ori(f1) - lacZ - T7 promoter - MCS (KpnI-SacI) - T3 promoter - lacI - ori(pMB1) - ampR - ori (2 μm) - ura3 [47]
 pRSXIPa pRS426; pTDH1-XIPa-tTDH This work
 pRSXI2.0 pRS426; pTDH1-XI2.0-tTDH This work
 pRSXIOrp pRS426; pTDH1-XIOrp-tTDH [40]