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Fig. 6 | BMC Biotechnology

Fig. 6

From: Effect of acetic acid on ethanol production by Zymomonas mobilis mutant strains through continuous adaptation

Fig. 6

RT-PCR detection of ZMO0117 and ZMO0119 mRNA from the acetate-tolerant mutant ZMA-142, ZMA-167 and ZM481. a Total RNA from the acetate-tolerant mutant ZMA-142(treated with 196 mM sodium acetate)(lane 2, 6, 10 and 14), ZMA-167 (treated with 196 mM sodium acetate)(lane 3, 7, 11 and 15) and ZM481 (without treated with sodium acetate) (lane 1, 5, 9 and 13) cells in the middle exponential phase were RT-PCR performed with various combinations of primers (ZMO0117F, ZMO0117R, ZMO0119F and ZMO0119R, respectively), in three independent reactions. Ten micrograms of each RNA was electrophoresed through 1% agarose–2.2 M formaldehyde gels and stained with ethidium bromide. The negative (lane 4, 8, 12, 16) controls sterile water. Lane M contained 0.1 ~ 3 kb markers. b Schematic representation of the ZM481, ZMA-142 and ZMA-167 chromosome containing the ZMO0117 and ZMI0119 genes. Arrows indicate positions of the predicted transcripts, B1 (transcipted from ZMO0117 to ZMO0119), B2 (ZMO0117), and B3(ZMO0119)

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BMC Biotechnology

ISSN: 1472-6750

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