Fig. 5

The Rho-independent terminator between the ZMO0117 and ZMI0119 genes in the Z. mobilis ZM4. a The genomic organization of ZMO0117 (ORF is shown in dark grey) and ZMO0119 (ORF is shown in light grey) was presented. b The translation start sites of the both genes as identified by genome annotation were marked with asterisks. The conserved Rho-independent terminator was predicted by TransTermHP server and shaded in color grey. The sequence was predicted as the putative promoter region of ZMO0119 using the Neutral Network Promoter Prediction (NNPP) server and underlined in black. The color black of square brackets indicated the regions of DNA deleted in the ZMA-167 in this study comparison with genome of ZM4, ZM481 and ZMA-142. The primer sequences used were double-underlined. c The schematic of the Rho-independent terminator motif for which TransTermHP search (HP score is −3.141 and 100% confidence). The terminators conserved sequence consist of a short stem-loop hairpin (standard Watson-Crick pairs and Wobble base pairs G-U shown as the grey and dotted lines, respectively) followed by thymine-rich region on their 3′ side