Fig. 2From: Comprehensive in silico allergenicity assessment of novel protein engineered chimeric Cry proteins for safe deployment in cropsIn vitro assessment of the chimeric proteins for allergenic potential. Analysis of the chimeric proteins for thermal stability: a and b SDS PAGE and Western blot analysis respectively showing stability of Cry1AcF and Cry1Aabc proteins after incubation at 100 °C for different time intervals. Lane 1- Cry1AcF without incubation, Lane 2–10 min incubation, Lane 3–30 min incubation, Lane 4–60 min incubation, M-Marker, Lane 5-Cry1Aabc without incubation, Lane 6–10 min incubation, Lane 7–30 min incubation, Lane 8–60 min incubation. Analysis of the chimeric proteins for pepsin digestibility: c and d SDS PAGE for in vitro digestibility of Cry1AcF protein in SIF (simulated intestinal fluid) with pepsin at pH 1.2 and 2.0 respectively and incubated at 37 °C for different time periods. e and f SDS PAGE for in vitro digestibility of Cry1Aabc protein in SIF with pepsin at pH 1.2 and 2.0 respectively and incubated at 37 °C for different time periods. (M- Marker, Lane 1–0 s incubation, Lane 2–30s incubation, Lane 3–60s incubation, Lane 4-5 min incubation, Lane 5–10 min incubation, Lane 6–30 min incubation, Lane 7- test protein with pepsin and without SIF and 30 min incubation, Lane 8- test protein with SIF and without pepsin and 30 min incubation, Lane 9- Intact test protein with 30 min incubation)Back to article page