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Fig. 2 | BMC Biotechnology

Fig. 2

From: Comprehensive in silico allergenicity assessment of novel protein engineered chimeric Cry proteins for safe deployment in crops

Fig. 2

In vitro assessment of the chimeric proteins for allergenic potential. Analysis of the chimeric proteins for thermal stability: a and b SDS PAGE and Western blot analysis respectively showing stability of Cry1AcF and Cry1Aabc proteins after incubation at 100 °C for different time intervals. Lane 1- Cry1AcF without incubation, Lane 2–10 min incubation, Lane 3–30 min incubation, Lane 4–60 min incubation, M-Marker, Lane 5-Cry1Aabc without incubation, Lane 6–10 min incubation, Lane 7–30 min incubation, Lane 8–60 min incubation. Analysis of the chimeric proteins for pepsin digestibility: c and d SDS PAGE for in vitro digestibility of Cry1AcF protein in SIF (simulated intestinal fluid) with pepsin at pH 1.2 and 2.0 respectively and incubated at 37 °C for different time periods. e and f SDS PAGE for in vitro digestibility of Cry1Aabc protein in SIF with pepsin at pH 1.2 and 2.0 respectively and incubated at 37 °C for different time periods. (M- Marker, Lane 1–0 s incubation, Lane 2–30s incubation, Lane 3–60s incubation, Lane 4-5 min incubation, Lane 5–10 min incubation, Lane 6–30 min incubation, Lane 7- test protein with pepsin and without SIF and 30 min incubation, Lane 8- test protein with SIF and without pepsin and 30 min incubation, Lane 9- Intact test protein with 30 min incubation)

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