Table 4 Oligonucleotides used in this study
From: An att site-based recombination reporter system for genome engineering and synthetic DNA assembly
Oligonucleotide | Sequence 5′ – 3′ |
|---|---|
PhiC31 Int F | ATGTACTAATCTAGAGAAGAGGATCAGAAATGGACACGTACGCGGGTGC |
PhiC31 Int R | CAAGCTTGCATGCCTGCAGG |
JB13 | AGCGGGTGTTCCTTCTTCACTG |
JB485 | TCTTCTCTAGATTAGTACATGCAACCA |
JB486 | CGACTAGAGTCGACCTGCAGCCAAGCTTAGTAAAGCCCTC |
MV26 | ACGGCTGACATGGGAATTGC |
MV143 | CCTCTTACGTGCCGATCAACGTCTC |
MV145 | GCTGGTGATTCCGCTTTGCGACTCAACCTTTTTCACCTAAAGTGCACCGACCGTGA |
MV146 | ACATCAGCGATCACCTGGCAGAC |
attBHKwtERI | AATTCCGCTTTGCGACTCAACCTTTTTCACCTAAAGTGCACCGACCGTGAATG |
attBHKwtREV | GATCCATTCACGGTCGGTGCACTTTAGGTGAAAAAGGTTGAGTCGCAAAGCGG |
attBHKmutERI | AATTCCGCTTTGCGACACAACCTTTTTCACCTAAAGTGCACCGACCGTGAATG |
attBHKmutREV | GATCCATTCACGGTCGGTGCACTTTAGGTGAAAAAGGTTGTGTCGCAAAGCGG |
40wtERI | AATTCTGCGACTCAACCTTTTTCACCTAAAGTGCACCG |
40wtREV | GATCCCGGTGCACTTTAGGTGAAAAAGGTTGAGTCGCAG |
40attBHKmutERI | AATTCTGCGACACAACCTTTTTCACCTAAAGTGCACCG |
40attBHKmutREV | GATCCCGGTGCACTTTAGGTGAAAAAGGTTGTGTCGCAG |
30wtERI | AATTCTCAACCTTTTTCACCTAAAGTG |
30wtREV | GATCCACTTTAGGTGAAAAAGGTTGAG |
30attBHKmutERI | AATTCACAACCTTTTTCACCTAAAGTG |
30attBHKmutREV | GATCCACTTTAGGTGAAAAAGGTTGTG |
30attBHKamp2ORF1min | TTTGCTCACAACCTTTTTCACCTAAAGTGGCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTT |
30attBHKamp1ORF1min | CTTTCACCAGCGTTTCTGGGTGCCACTTTAGGTGAAAAAGGTTGTGAGCAAAAACAGGAAGGCAAAATGCCGC |
30attBHKamp2ORF2min | TTTGCTACACAACCTTTTTCACCTAAAGTGCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTT |
30attBHKamp1ORF2min | CTTTCACCAGCGTTTCTGGGTGCACTTTAGGTGAAAAAGGTTGTGTAGCAAAAACAGGAAGGCAAAATGCCGC |
30attBHKamp2ORF3min | TTTGCTGCCACTTTAGGTGAAAAAGGTTGTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTT |
30attBHKamp1ORF3min | CTTTCACCAGCGTTTCTGGGTGACAACCTTTTTCACCTAAAGTGGCAGCAAAAACAGGAAGGCAAAATGCCGC |
phiC31 ORF1 F | TTTGCTTGCGGGTGCCAGGGCGTGCCCTTGGGCTCCCCGGGCGCGTACTCCCACCCAGAAACGCTGGTGAAAG |
phiC31 ORF2 F | TTTGCTGCGGGTGCCAGGGCGTGCCCTTGGGCTCCCCGGGCGCGTACTCCCCACCCAGAAACGCTGGTGAAAG |
phiC31 ORF3 F | TTTGCTCGGGTGCCAGGGCGTGCCCTTGGGCTCCCCGGGCGCGTACTCCCCCACCCAGAAACGCTGGTGAAAG |
phiC31 ORF4 F | TTTGCTGGAGTACGCGCCCGGGGAGCCCAAGGGCACGCCCTGGCACCCGCACACCCAGAAACGCTGGTGAAAG |
phiC31 ORF5 F | TTTGCTGGGAGTACGCGCCCGGGGAGCCCAAGGGCACGCCCTGGCACCCGCCACCCAGAAACGCTGGTGAAAG |
phiC31 ORF6 F | TTTGCTGGGGAGTACGCGCCCGGGGAGCCCAAGGGCACGCCCTGGCACCCGCACCCAGAAACGCTGGTGAAAG |
phiC31 ORF1 R | CTTTCACCAGCGTTTCTGGGTGGGAGTACGCGCCCGGGGAGCCCAAGGGCACGCCCTGGCACCCGCAAGCAAAAACAGGAAGGCAAAATG |
phiC31 ORF2 R | CTTTCACCAGCGTTTCTGGGTGGGGAGTACGCGCCCGGGGAGCCCAAGGGCACGCCCTGGCACCCGCAGCAAAAACAGGAAGGCAAAATG |
phiC31 ORF3 R | CTTTCACCAGCGTTTCTGGGTGGGGGAGTACGCGCCCGGGGAGCCCAAGGGCACGCCCTGGCACCCGAGCAAAAACAGGAAGGCAAAATG |
phiC31 ORF4 R | CTTTCACCAGCGTTTCTGGGTGTGCGGGTGCCAGGGCGTGCCCTTGGGCTCCCCGGGCGCGTACTCCAGCAAAAACAGGAAGGCAAAATG |
phiC31 ORF5 R | CTTTCACCAGCGTTTCTGGGTGGCGGGTGCCAGGGCGTGCCCTTGGGCTCCCCGGGCGCGTACTCCCAGCAAAAACAGGAAGGCAAAATG |
phiC31 ORF6 R | CTTTCACCAGCGTTTCTGGGTGCGGGTGCCAGGGCGTGCCCTTGGGCTCCCCGGGCGCGTACTCCCCAGCAAAAACAGGAAGGCAAAATG |