Table 3 Plasmids used in this study
From: An att site-based recombination reporter system for genome engineering and synthetic DNA assembly
Name | Description | Reference/Source |
|---|---|---|
pSW23T | pSW23::oriTRP4; [CmR]; oriVR6K | [29] |
pSU38Δ | orip15A [KmR] | [48] |
pHK-Int | pGB2ts::cI857-λ-PR-HKInt, [SpR] | [30] |
pHK11-Amp | pLDR11::attP_HK, [ApR,TcR] | [30] |
pSC101 | pSC101ts, repA [TcR] | [49] |
pUC19 | oriColE1, lacZα [ApR] | [50] |
pBAD43 | oripSC101, PBAD::MCS,[SpR] | [51] |
pHK11Δamp | pHK11-Amp::attP_HK,ΔAmp, [TcR] | this study |
pMP96 | pSC101ts::cI857-λ-PR-(HKXis-HKInt λXis-λInt), [SpR] | [23] |
pMP58 | pSC101ts::oriTRP4;repA, [CmR,ApR] | this study |
pMDG1 | pMP58;bla::attB_HK,[ApR,CmR] | this study |
pMDG2 | pSW23T::bla::attB_HK from pMDG1 | this study |
pMDG3 | α/pSU38::attR_HK, [ApR] | this study |
pMDG4 | pSW23T::attL_HK, [CmR] | this study |
pMJM1 | pSW23T::attB_HKwt, [CmR] | this study |
pMJM2 | pSW23T::attL_HKmut, [CmR] | this study |
pMJM3 | pSW23T::attL_HK40, [CmR] | this study |
pMJM4 | pSW23T::attL_HK30, [CmR] | this study |
pJB6 | pSU38Δ::attR_HK-attL_λ, [ApR] | this study |
pJB7 | pSW23T::attR_HK-attL_λ, [CmR] | this study |
pJB8 | pBAD43::HKXis-HKInt λXis-λInt, [SpR] | this study |
pZJ7 | pBAD33::ΦC31Int, [CmR] | J. Zhao and S. Colloms |
pZJ7ΔXbaI | pZJ7 with SpeI – XbaI fragment deleted | this study |
pPhiC31-Int | pGB2ts::cI857-λ-PR-ΦC31Int, [SpR] | this study |
pPhiC31-attP | pHK11Δamp::attP_ΦC31, [TcR] | this study |