Table 2 Bacterial strains used in this study
From: An att site-based recombination reporter system for genome engineering and synthetic DNA assembly
E. coli | Â | Â |
|---|---|---|
Name | Genotype | Reference/Source |
β2163 | (F−) RP4–2-Tc::Mu ΔdapA::(erm-pir) [KmR EmR] | [29] |
π1 | DH5αΔthyA::(erm-pir116) [EmR] | [29] |
MFDpir | MG1655 RP4–2-TC::[Mu1::aac(3)IV-ΔaphA-Δnic35-ΔMu2::zeo] ΔdapA::(erm-pir)ΔrecA[ApraR ZeoRErmR] | [47] |
PGB-8557 | DH5α strain containing plasmids pHKΔ-Amp and pHK-Int [TcR SpR] | this study |
PGB-E274 | DH5α strain containing plasmids pΦC31-attP and pΦC31-Int [TcR SpR] | this study |
One Shot ® Top10 | F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 Δ lacX74 recA1 araD139 Δ(araleu)7697 galU galK rpsL (StrR) endA1 nupG | ThermoFisher Scientific |