Fig. 9From: Site-specific chemical conjugation of human Fas ligand extracellular domain using trans-cyclooctene – methyltetrazine reactionsIsolation of rFab’-hFasLECD conjugates by high-performance size-exclusion chromatography. Panels: a and c, reaction mixture after quenching with MTZ-PEG4-Amine. (a, 1.0 M excess amount of rFab’-MTZ; c, 5.0 M excess amount of rFab’-MTZ); b, isolated sample from a; d, isolated sample from c. Retention time of each peak is shownBack to article page