Fig. 6From: Site-specific chemical conjugation of human Fas ligand extracellular domain using trans-cyclooctene – methyltetrazine reactionsConjugation reaction of Avidin-MTZ and hFasLECD-TCO analyzed by high-performance size-exclusion chromatography. Panels: a, Avidin-MTZ alone; b–e, reaction mixtures (b: 1.0, c: 1.2, d: 1.5 and e: 3.0 M excess amounts of Avidin-MTZ were reacted with hFasLECD-TCO). Retention time of each peak is shownBack to article page