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Fig. 5 | BMC Biotechnology

Fig. 5

From: Site-specific chemical conjugation of human Fas ligand extracellular domain using trans-cyclooctene – methyltetrazine reactions

Fig. 5

Complex formation of sulfo-Cy3 conjugated hFasLECDs with hFasRECD-Fc. L and R indicate the positions of sulfo-Cy3 conjugated hFasLECDs and hFasRECD-Fc, respectively. a SDS-PAGE analysis of the receptor mediated co-immunoprecipitation. Lanes: M, molecular-weight size markers; 1 and 2, sulfo-Cy3-MT-hFasLECD; 3 and 4, sulfo-Cy3-TM-hFasLECD; 5 and 6, buffer; 1 and 3, purified samples; 5, buffer alone sample; 2, 4 and 6, co-immunoprecipitated materials. b High-performance size-exclusion chromatography profiles. The mixtures of sulfo-Cy3 conjugated hFasLECDs (7.5 μg each) and hFasRECD-Fc (19.4 μg each) were analyzed. Upper panel, sulfo-Cy3-MT-hFasLECD; lower panel, sulfo-Cy3-TM-hFasLECD

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