Fig. 4

Spectroscopic analysis of sulfo-Cy3 conjugated hFasLECDs. Dot lines, Sulfo-Cy3-MT-hFasLECD; solid lines, Sulfo-Cy3-TM-hFasLECD. a UV-Vis spectra. The absorbance of Sulfo-Cy3-MT-hFasLECD was expressed as values of the experimental data plus 0.2. Insert, an appearance under white light. b Fluorescence emission spectra excited at 552 nm. The relative fluorescence intensity of Sulfo-Cy3-MT-hFasLECD was expressed as values of the experimental data plus 20. Insert, a fluorescence emission observed in the measurement cuvette