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Fig. 3 | BMC Biotechnology

Fig. 3

From: Site-specific chemical conjugation of human Fas ligand extracellular domain using trans-cyclooctene – methyltetrazine reactions

Fig. 3

Conjugation of hFasLECD with sulfo-Cy3. a SDS-PAGE analysis of the conjugation reaction. Lanes: M, molecular-weight size markers; 1, hFasLECD-MTZ alone; 2, hFasLECD-MTZ reacted with a 1.3 M excess amount of sulfo-Cy3-TCO; 3, hFasLECD-TCO alone; 4, hFasLECD-TCO reacted with a 1.4 M excess amount of sulfo-Cy3-MTZ. b High-performance size-exclusion chromatography profiles. Upper panels, hFasLECD-TCO reacted with a 1.4 M excess amount of sulfo-Cy3-MTZ; lower panels, hFasLECD-MTZ reacted with a 1.3 M excess amount of sulfo-Cy3-TCO; left panels, crude samples; right panels, purified samples

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BMC Biotechnology

ISSN: 1472-6750

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